ToFU2¶
Description¶
This is the developmenal (ToFU2) branch of the PacBio IsoSeq analysis software. The isoform sequencing (Iso-Seq) application generates full-length cDNA sequences — from the 5’ end of transcripts to the poly-A tail — eliminating the need for transcriptome reconstruction using isoform-inference algorithms. The Iso-Seq method generates accurate information about alternatively spliced exons and transcriptional start sites. It also delivers information about poly-adenylation sites for transcripts up to 10 kb in length across the full complement of isoforms within targeted genes or the entire transcriptome.
Environment Modules¶
Run module spider tofu2
to find out what environment modules are available for this application.
Environment Variables¶
- HPC_TOFU2_BIN - executable directory
Categories¶
biology, ngs, sequencing