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SCRIPTURE

Description

scripture website

Scripture is a method for transcriptome reconstruction that relies solely on RNA-Seq reads and an assembled genome to build a transcriptome ab initio. The statistical methods to estimate read coverage significance are also applicable to other sequencing data. Scripture also has modules for ChIP-Seq peak calling. Note: export _JAVA_OPTIONS="-Xmx?g" where ? equals to the available memory.

Environment Modules

Run module spider scripture to find out what environment modules are available for this application.

Environment Variables

  • HPC_SCRIPTURE_DIR - installation directory
  • HPC_SCRIPTURE_BIN - executable directory
  • HPC_SCRIPTURE_LIB - library directory

Citation

If you publish research that uses scripture you have to cite it as follows:

Ab initio reconstruction of cell type–specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs

Categories

biology, genomics