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RSeQC

Description

rseqc website

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. “Basic modules” quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while “RNA-seq specific modules” investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation

Environment Modules

Run module spider rseqc to find out what environment modules are available for this application.

Environment Variables

  • HPC_RSEQC_DIR - installation directory
  • HPC_RSEQC_BIN - executable directory

Citation

If you publish research that uses rseqc you have to cite it as follows:

Wang L, Wang S, Li W* RSeQC: quality control of RNA-seq experiments Bioinformatics (2012) 28 (16): 2184-2185. doi: 10.1093/bioinformatics/bts356 pubmed

Categories

biology, ngs, rna-seq