mOTU¶
Description¶
mOUT is a method that uses marker genes for taxonomic profiling of metagenomes. Phylogenetic markers are genes that can be used to reconstruct the evolutionary history of organisms and to profile the taxonomic composition of environmental samples. Efforts to find a good set of protein-coding phylogenetic marker genes (Ciccarelli et al., Science, 2006; Sorek et al., Science, 2007) led to the identification of 40 universal marker genes (MGs). These 40 MGs occcur in single copy in the vast majority of known organisms and they have recently been used to delineate prokaryotic organisms at the species level (Mende et al., 2013). directory and adjust it before running ruMOCAT.sh
Environment Modules¶
Run module spider motu
to find out what environment modules are available for this application.
Environment Variables¶
Note: Copy the MOCAT config from $HPC_MOCAT_CONF/MOCAT.cfg to the working * HPC_MOTU_DIR - installation directory * HPC_MOTU_BIN - executable directory * HPC_MOTU_DOC - documentation directory
Citation¶
If you publish research that uses motu you have to cite it as follows:
Sunagawa et al., Nature Methods, 2013.
Categories¶
biology, genomics