Usage

Metabin uses Jim Kent's Blat application as an alignment method that is much faster than Blastx. Unfortunately, Blat has a bug which causes it to crash with large reference databases, like nr (see the Blat Wiki Page). As a workaround, we suggest dividing large reference databases into multiple files. To implement this, it is necessary to run the prepareinput step of Metabin with the -b n option, and run the Blat analyses separately.