IsoSeq3

Description

isoseq3 website

IsoSeq - Scalable De Novo Isoform Discovery from Single-Molecule PacBio Reads. IsoSeq contains the newest tools to identify transcripts in PacBio single-molecule sequencing data. Starting in SMRT Link v6.0.0, those tools power the IsoSeq GUI-based analysis application. A composable workflow of existing tools and algorithms, combined with new clustering techniques, allows users to process the ever-increasing yield of PacBio machines. Starting with version 3.4, support for UMI and cell barcode based deduplication has been added. Version 4.0 adds a new cluster2 tool that enables clustering of hundreds of millions of HiFi reads. Note: Isoseq3 has been renamed to Isoseq to enable major version changes.

Environment Modules

Run module spider isoseq3 to find out what environment modules are available for this application.

Environment Variables

• HPC_ISOSEQ3_DIR - installation directory
• HPC_ISOSEQ3_BIN - executable directory

Categories

biology, ngs